Tuberculosis is the contagious disease caused by microorganism called “Mycobacterium tuberculosis”. It remains one of the deadliest bacterial infections globally, with raising incidences of multidrug-resistant (MDR), extensively drug-resistant (XDR) and totally drug- resistant (TDR) strains posing critical treatment challenges. Fluoroquinolones are anti-microbial, that inhibits the DNA gyrase A and B enzyme. Despite its efficacy, the limitations of Flu, including potential cardiac toxicity and phospholipidosis, highlight the urgent need for safer and more potent analogs. This review describes how computational methods like molecular docking and protein optimization plays a crucial role in enhancing the effectiveness and safety profiles of anti-TB drugs

This review focuses on the development and validation of UV Spectrophotometric methods for the analysis of mentioned cardiovascular drugs. It provides an overview of various UV spectroscopic techniques employed for the estimation of cardiovascular drugs in pharmaceutical formulations. The review discusses the key aspects of method development, including wavelength selection, and optimization of experimental conditions. Additionally, it highlights parameters such as linearity, accuracy, precision, specificity, and robustness. The review aim to provide a comprehensive summary of the UV spectroscopic methods developed and validated for cardiovascular drugs, highlighting their advantages, limitations and applications in pharmaceutical analysis

The development and testing of a transdermal film-forming gel (TFFG) impregnated with terbinafine to treat superficial fungal infections including athlete foot, ringworm and onychomycosis. Terbinafine is an antifungal agent made synthetically, which is known to inhibit fungal growth, although, when administered orally, it usually results in other side effects such as liver toxicity and gastrointestinal disturbances. To address such concerns, a formulation of TFFG was designed with the purpose of delivering the drug to the site of infection and reducing systemic absorption and side effects. Hydroxypropyl methylcellulose (HPMC) and Eudragit polymers were used to prepare the gel in the presence of ethanol, as a penetration enhancer to ensure the absorption of the drug through the skin. Some of the properties that were considered in the development of the formulation include pH, viscosity, spreadability, drug content, drying time, film thickness and antifungal activity. The findings revealed that the gel had good uniformity in drug content (88.08% 97.14%), satisfactory spreadability (4.15 cm 5.78 cm) and controlled rate of release. Formulation F10 containing 15 percent Eudragit RS100 showed the most favorable sustained release and antifungal release, thus a potential contender in the treatment of chronic fungal infections. This is a safe and effective patient-friendly alternative to oral therapies

RP-HPLC was developed and validated for the measurement of Canagliflozin in tablet dosage forms. Canagliflozin, a type 2 diabetes mellitus (T2DM) inhibitor, requires precise analysis in order to ensure quality and consistency. Based on the optimal method, methanol, water, and acetonitrile were used as mobile phases in a ratio of 40:40:20, with 225 nm detection. There was excellent linearity, with a correlation coefficient (r2) of 0.999, precision (intra-day RSD = 0.435%, inter-day RSD = 1.033%), and accuracy (recovery between 99.1% and 100.2%). Limits of detection (LOD) and limits of quantification (LOQ) were 0.34 µg/mL and 1.03 µg/mL, respectively. Flow rate, composition of the mobile phase, and wavelength of the detection were not sensitive to changes in the method. As a routine quality control and stability test method for Canagliflozin in bulk and finished dosage forms, this RP-HPLC method is suitable. As a result of the validation, the system is confirmed to be capable of conforming to regulatory standards for pharmaceutical analysis