The present study investigates the presence of phytochemical compounds in the chloroform extract of Phyllanthus acidus L, employed by Gas chromatography-Mass Spectrometry. 16 bioactive phytocompounds were found in the chloroform extract of Phyllanthus acidus. The prevailing compounds were (15.221%) of Z-5,17-octadecadien-1-ol acetate, followed by Tritetracontane (13.224%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol(11.730%), Octadecanoic acid, ethyl ester (7.962%), vitamin E (6.197%), Dotriacontane (4.118%) which has medicinal properties such as Antioxidant, Antitumor, analgesic, antibacterial, anti-inflammatory, sedative, fungicide, Hypocholesterolemic, Nematicide, Pesticide, Lubricant. ,Antimicrobial, are used for the treatment of several ailments

Chromatography with high performance consists basically of a column filled with packing material (stationary phase), a pump that moves the mobile phase(s) through the column, and a detector that detects the molecule's retention time. Gemigliptin, sold as Zemiglo, is an oral hypoglycemic agent (anti-diabetic drug) that is a member of the dipeptidyl peptidase- 4 (DPP-4) inhibitor class of medications. Thus, the goal of this work is to develop a novel analytical approach for determining Gimagliptin concentrations using RPHPLC. As the mobile phase, a mixture of acetonitrile and water with various compositions was utilised at a flow rate of 1,1.2ml/min. The stationary phase was a phenomenex C18 column. Numerous parameters such as accuracy, precision, linearity, system suitability, and formulation assay were examined, and an analytical method for qualitative and quantitative drug estimation was established

Colorectal cancer is the third highest pervasive rate of all cancer types worldwide and it is also one of the major causes of mortality. Chemotherapy drugs are generally limited due to various complexities, as well as the development of resistance and recurrence. Hence, scientific communities have given more significance to natural products as drugs derived from them provide better treatment compared to synthetic product. Besides, the availability of compounds derived from natural sources, especially the secondary metabolites derived from microorganism play important role in cancer treatment. In these Soil grampositive bacteria streptomyces colonosanans (CA-256286) secondary metabolites has been known an attractive compound with the ability of anticancer activity in cancer cells. In the present study, the in-silico docking examination involved exploration of protein, 3D Structural modelling and binding energy calculation. SMAD2 (PDB id: 1 KHX) Protein was used in the molecular docking analysis of secondary metabolites such as 1,8 dihydroxy-2-ethyl-3-methyl anthraquinone, anthracycline, elaiomycin, piericidin, streptokordin for chemotherapeutic activity. The compound anthracycline had the highest binding energy scores with the target protein SMAD2 (PDB id: 1 KHX), according to molecular docking results. The findings suggest it could be used to develop new anticancer drugs. The conclusion drawn from the study should be validated by further evaluation in animals and humans

Mimosa pudica is a plant species under the family fabaceae which have been used as a folk medicine in curing assorted ailments. The endeavor of this study was to quantify, isolate and characterize the therapeutically important bioactive constituent ?-sitosterol present in n-hexane extract of whole plant of Mimosa pudica. The HPTLC scrutiny was done to quantify the amount of ?-sitosterol in whole plant extract, which yields 2.901 mg/g. Further, isolation was carried out by preparative TLC with reference standard. The compound was scrapped in accordance with the Rf value of standard. The physical and chemical properties of isolated compound were compared with standard ?-sitosterol for identification. The UV spectroscopy, Fourier-transform infrared spectroscopy, 1H NMR and 13C NMR analysis was carried out for characterization of isolated compound. The interpretation data obtained through spectral analysis shows the nature of carbon and hydrogen atoms which confirms that the isolated compound was ?-sitosterol.

Kabasura Kudineer is the siddha formulation which is in the form of powder, capsule, tablet etc. These formulations consist of 13 to 15 different plant extract which shows the suitable efficacy. It is used to treat fever and respiratory problem. Thus, it has Anti-oxidant, Anti-inflammatory, Anti-pyretic property. During the previous outbreak of dengue, chikungunya and swine flu in Tamil Nadu Kabasura kudineer were used to control the dreadful disease. The present pandemic has increased the public attention towards the benefits of Kabasura kudineer in Tamil Nadu. It is used in COVID 19 as prophylactic and as a treatment. There are various commercially available of Kabasura kudineer in market. To check its standards, here we conduct different monograph analysis for some available marketed brands compare with the standard reference. The physicochemical and phytochemical characterization of the samples was carried out in accordance with the standards laid down by Indian Pharmacopoeia (IP) 2018. HPTLC profiling of key constituents including phenol, flavonoids, tannin, steroids and alkaloids were also carried out in accordance with IP 2018 monographs. The chromatographic analysis showed the presence of all major ingredients in both commercially available sample and the standard and all the physicochemical and phytochemical properties were also found similar among preparations. Our findings may boost the global recognition of Kabasura kudineer is used to increase the immune system

Any physiological or psychological demand causes the body to respond in a non-specific manner to stress. Stress-induced neurochemical and behavioral deficits have been shown in preclinical studies. Haloperidol was used to induce acute parkinsonian-like effects in rats in the present study, which examined the hypothesis that stress adaptation would reduce the responsiveness of somatodendritic 5-hydroxytryptamine (5-HT)-1A receptors. After just a single immobilization stressful events of 120 minutes, there have been negative effects on food consumption, overall growth, and motor performance; however, subsequent immobilization stress exposure did not result in these stress-induced behavioural abnormalities (120 minutes per day for 6 days).This suggests that behavioral tolerance to similar stress occurs. Compared to their respective control animals, animals exposed to repeat immobilization stress showed reversal of the haloperidol-induced motor deficits. Dopamine system could be released from inhibitory effects of serotonin induced by stress by desensitizing somatodendritic 5- HT-1A and 5-HT-2C receptors. Increasing 5-HT-1A receptor activity modulates dopaminergic neuron activity directly and may reverse haloperidol-induced parkinsonian symptoms in repeated-immobilized rats with haloperidol