Ambroxol hydrochloride and Doxofylline are having π electrons in their structure and hence absorbs electromagnetic radiation in the UV region. This character of the drugs are used for estimating them in combination by UV-spectroscopic method using Jasco V-530 double beam UV-Vspectrophotometer, The calibration curves were obtained for both Ambroxol hydrochloride and Doxofylline in the range of 5-50μg/ml and 40-400μg/ml, respectively. The slope, intercept and correlation coefficient values of Ambroxol hydrochloride and Doxofylline at 249nm were found to be 0.0221, 0.0187 and 0.9983 and 0.0003, 0.0250, and 0.9996at 275nm were found to be 0.0019, 0.0137 and 0.9977 and 0.0032, -0.0014 and 0.9995, respectively. In RP-HPLC Ambroxol hydrochloride and Doxofylline system gave symmetric peak shapes, and minimum of tailing. The retention time for Ambroxol hydrochloride and Doxofylline were found to be 2.4 and 3.7 minutes, respectively. Linearity of Ambroxol hydrochloride and Doxofylline were found to be 1-10 μg/ml for both drugs and correlation coefficient value was found to be 0.999 and 0.997, respectively showing good correlation between concentration and peak area response. The LOD and LOQ of Ambroxol hydrochloride and Doxofylline were found to be 50 and 500 ng/ml respectively for both drugs.

1,3-Indanedione is an organic compound is the molecular formula C9H6O2. Chemically, it is β-diketone 1,3-Indanedione, a
powerful catalyst, with anticoagulant activity with aromatic core has become prominent in pharmacy during the year, 1,3-
Indandione has become an important part of multifaceted reactions in the development of various compounds, , minerals.
Anticoagulants, commonly known as anticoagulants, are chemical substances that prevent or reduce blood clotting and
prolong the clotting time. Research on the literature revealed that derivatives of indan 1,3 dione, in particular 2-arylsulfonyl
indan-1,3 dione, have been found to be potent blood anticoagulants. Previously, they were prepared by sealing Claisen
containing diethyl phthalate and arylmethylsulfon by methods analogous to the production of indan-1,3-dione from diethyl
phthalate and ethyl acetoacetate. In search of improved oral anticoagulants, 1, 3-dione in a different way involving
Knoevenagel reaction between phthalin anhydride and arylsulfonyl acetate. The reaction is based on the observation that
phthalin anhydride can act as a carbonyl compound in Perkin-type reactions. This article is a summary of indane 1,3 dione as
anticoagulant agents