This  presented  work  is  based  on  application  of  two  multivariate  calibration  methods  for  simultaneous  UV-Vis spectrophotometric determination of active substances in combined pharmaceutical formulation composed of Cefixime (CEF) and Ornidazole (ONZ). The methods used were Principal Component Regression (PCR) and Partial Least Square (PLS). The Spectra  of  CEF  and  ONZ  were  recorded  at  concentrations  within  their  linear  ranges  2.0-12.0  μg/ml  and  5.0-30.0  μg/ml, respectively. 27 set of mixtures were used  for calibration and 9 set of mixtures were used for validation in the wavelength range  of  260  to  330  nm  with  the  wavelengths  intervals  λ=  0.5  nm  in  methanol.  The  methods  were  validated  as  per International  Conference  on  HarmonizationQ2  (R1)  (ICH)  guidelines.  These  methods  were  successfully  applied  for determination  of  drugs  in  pharmaceutical  formulation  (tablet)  with  no  interference  of  the  excipients  as  indicated  by  the recovery study results. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality controlas well as in process control of drugs and formulation.

The objective of this study was to investigate the in vitro antioxidant activity of hydro alcoholic extract of the stem Acacia torta (Roxb.) Craib using DPPH radical scavenging assay and concentration was being (IC50 135.9 μg/ml). Moreover, the saponins, steroids, triterpenes, alkaloids and tannins were also present in the hydro alcoholic extract of stem Acacia torta. The characterization of the extract was also performed by HPLC profiling with the standard, Quercetin. The present study demonstrated that Acacia torta (Roxb.) Craib stem extract exhibited potent antioxidants in DPPH free radical assay as compared to standard quercetin. Keywords: Acacia torta (Roxb.) Craib, DPPH radical scavenging, Antioxidant.

The methanolic-HCl extract of Beta vulgaris was studied for the cardioprotective activity using dexamethasone induced cardiotoxicity. Methanolic-HCl extract of Beta vulgaris was shown significant cardioprotective activity by elevating antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH) and Lipid peroxidation (LPO). The phytochemical present in Beta vulgaris like Alkaloids, glycosides, tannin, flavonoids and betacyanin which is confirmed from qualitative analysis may be responsible for its cardioprotective activity. Keywords: Dexamethasone, Beta vulgaris, Cardiotoxicity.