ABSTRACT Zidovudine is widely used in ART as Nucleoside Reverse Transcriptase Inhibitors. In the present investigation an attempt has been made to design and develop Zidovudine S.R. tablets using various synthetic and natural hydrophilic polymers. Sustained release matrix tablets are prepared using Xanthan gum, HPMC K100M, Guar gum alone and in a combination by using direct compression method. A total of 7 formulations (3 formulations with Xanthan gum, HPMC, Guar gum respectively, 4 formulations containing HPMC and guar gum in combination with Xanthan gum in the ratio of 5:5 and 2.5:7.5 respectively) were prepared. All these prepared tablets are evaluated for weight variation, friability, thickness, hardness, drug content and drug release pattern. No significant variation from I.P. and U.S.P. limits was observed. The drug-excipient interaction studies were carried out by FTIR spectroscopy, DSC and visible inspection (by keeping drug excipient mixture for 1 month at room temperature). No significant interaction between rug and excipients was observed. The comparative evaluations of the dissolution profiles were done. From the data it was concluded that all formulations show Fickian diffusion or anomalous type drug release pattern. Of all the formulations F6 is the best formulation .From the above, it was concluded that few of the formulations (F1, F5, F6, and F7) follow the sustained release pattern of drug release. Keywords: Zudovudine, Synthetic & natural polymers, In-vitro Evaluation, S.R Matrix Tablets.

ABSTRACT Both the plants, A. paniculata and C. igneus are easily grown in the kitchen garden of the back yard. Their phenolic contents are responsible for their antioxidant properties. The present paper has been undertaken to consider pot experiments in the soil of Urga village of Korba district. As previous works have found the performance of genotypes in the growth and yield in some cases f medicinal plants. These plants, A. paniculata and C. igneus show pronounced phenolic contents and hence their free radicals scavenging activity. Keywords: Fly- ash, genotypes, protease activity 1,1 DPPH (Diphenyl 1-2 picryl Hydrazyl), Antioxidant activity, Atherosclerosis.

ABSTRACT Meloxicam is 4-hydroxy-2-methyl-N-(5-methyl-1,3-thiazol-2-yl)-2H-1,2-benzothiazine-3-carboxamide 1,1-dioxide is an selective to cyclooxygenase-2 (Cox-2) inhibitor and used in analgesic, antipyretic activities belongs to (NSAIDS)and in rheumatoid arthritis. Literature survey reveals that meloxicam is estimated individually by uv spectrometry, RP-HPLC, LC/MS, LC/MS/MS and stability indicating HPLC and TLC. Keywords: Meloxicam, RP-HPLC,LC/MS/MS.LC/MS.HPTLC,TLC.

ABSTRACT

Elucidation of structure-function relationships of seed storage proteins is a prerequisite for developing theoretically new food and/or food materials based on seed storage proteins. Furthermore, recent findings suggest that the 7S globulin, among all storage proteins in the coconut seeds, is responsible for the up-regulation of LDL receptors and that this activation is induced by the  α and αβ- subunits of 7S globulin. The molecular mechanism underlying this biological response is currently under investigation.  Here we report the purification and biophysical characterization of 7S globulin protein from the coconut endosperm. The total protein was separated by centrifugation at 13500g for 15 min at 4 ºC. The total precipitated protein of the 60% ammonium sulfate was subjected to column chromatography on a Hi-Prep 1.5/20 DEAE Sephadex A-50 HR column  at a flow rate of 1 mL/min followed by purification using gel filtration chromatography. The purified fractions will be analyzed by SDS-PAGE. The band of SDS PAGE was excised and identified by mass spectrometry. These fragments are analyzed through MASCOT and identified through BLAST searches.  We further perform circular dichroism (CD) analysis to  observe  the  effect  of  temperature  on  secondary  structure  of  the  protein  and thermal denaturation studies were carried out at 222 nm.  This study will provide a sound basis for understanding in the structure-function relationship of coconut protein especially basic 7S globulin.

 Keywords: Basic 7S Globulin, cocosin, Coconut Endosperm, Circular Dichroism, Mass Spectrometry, Cholesterol metabolism,  Dietary Proteins and  Seed Storage Proteins.

ABSTRACT Dispersed phase preparation is an important step in the formulation of suspensions. One of the good criteria for suspension is fine (micron) sized dispersed phase particles. Fine size of the particles in a suspension is required for good physical stability and rapid dissolution rate. The particle size in a suspension can be reduced by techniques such as by micronisation using various size reduction machineries and also by pharmaceutical techniques such as co-precipitation and pH change method. The objective of this study was to prepare and evaluate sulphamethoxazole suspension by pH change method. The plots of sedimentation volume versus time indicated slow settling of dispersed particles in the case of suspensions prepared by pH change method when compared to control formulations. These results indicated that the dispersed phase particles remain suspended over a longer period of time in the case of suspensions prepared by pH change method. The better suspendability of the particles in the suspensions prepared by pH change method is due to reduction in particle size. Keywords: Sulphamethoxazole, pH change method, Suspension, Sedimentation Volume, Micronisation, Physical stability

ABSTRACT The titled compounds (4a-4k) have been synthesized by the condensation of 5-{4'-[(3"-aryl)-2"-Propene-1"-one]-Phenyl carbamido}-dibenz [b,f] azepines with malononitrile and Sodium methoxide. The biological activities of these compounds have been determined against various Gram +ve, Gram –ve bacteria and fungi. The constitutions of the products are supported by IR, 1 H NMR, Mass spectra and elemental analysis. Keywords: Cyano pyridine derivaties, Antimicrobial, Azepines.

ABSTRACT The present research work discusses the development of UV Spectroscopic method for the estimation of Guaifenesin. Simple, specific, accurate and cost effective spectroscopic method has been developed for the estimation of Guaifenesin in bulk as well as formulation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λmax) was found to be 240nm. The validation was performed as per ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The method shows high sensitivity with linearity in the range of 1-9 μg/ml and shows a linear relationship between the absorbance and concentration with coefficient of correlation 0.9992. The regression of curve was Y = 0.054 + 0.030. The precision of method was found to be good. The percentage recovery was found to be 99.57 ± 0.38. The optimized showed good reproducibility and recovery with RSD < 2%. The proposed method will be suitable for analysis of Guaifenesin in bulk as well as pharmaceutical formulations in quality control purpose. It is thus concluded that the proposed method is new, simple, cost effective, safe, accurate, precise and environmental friendly. Keywords: Guaifenesin, UV Spectroscopic method, Sensitive, Validation, ICH guidelines.

ABSTRACT Agomelatine (N-[2-(7-methoxynaphthalen-1-yl) ethyl] acetamide), its antidepressant efficacy has been verified in the treatment of major depressive disorder (MDD).Agomelatine showed significant benefits over paroxetine due to the complete absence of side effects including the associated sexual side effects that are troublesome with some antidepressants. Agomelatine has also proven to have anxiolytic properties and thus may prove to be very useful in the treatment of anxiety disorders. Literature survey reveals that Agomelatine is estimated individually by uv spectrometry, RP-HPLC, LC-MS/MS , Stability indicating determination by HPLC-UV, Stability Under different stress Condition by HPLC-Fluoresence and TLC. Keywords: Agomelatine, RP-HPLC, LC-MS/MS.UV Spectrometric, HPLC-UV, HPLC, Fluoresence and TLC.

ABSTRACT A new stability indicating reversed phase HPLC method was developed and validated for assay of fusidic acid, betamethasone -17 valerate and Chlorocresol in Topical pharmaceutical formulation. The chromatographic separation was achieved on Lichrospher RP-18 125 mm x 4.0mm, 5 μm column ,Using a mobile phase comprising of mixture of Acetonitrile, Methanol and 0.05M Ortho-Phosphoric acid in the ratio of 50:5:45 (v/v), at a flow rate of 1.5 ml/min. Injection volume 10 μL, The column temperature was set at 25°C. The detection was carried out at 235 nm. The precision of the method observed in RSD is 0.5 %, 0.7 % and 0.4 %, The Overall RSD of Method Precision and Intermediate Precision are 0.9 %, 1.7 % and 2.3 % , Individual % recovery values observed in the range of 96.1 % to 101.6 %, 96.9 % to 103.9 % and 91.8 % to 99.4 % for Fusidic acid, Betamethasone-17 valerate and Chlorocresol respectively. Sample solution is observed to be stable at least 36 hours at room temperature. The method is found to be robust under the following variable conditions like flow + 10%, column oven temperature + 5°C, organic content in mobile phase + 2%, and wave length + 5 nm. The linearity of response was determined in the range of 289.96 μg/mL to 942.38 μg/mL for Fusidic acid, 19.01 μg/mL to 61.77 μg/mL for Betamethasone-17 valerate and 15.95 μg/mL to 51.85 μg/mL for Chlorocresol, the Correlation coefficient is 0.99886, 0.99898 and 0.99884 respectively. Significant degradation was observed during the Forced degradation studies, in drug product and placebo were exposed to 40°C / 15 minute in hydrolysis Acid, Alkali 1 N NaOH , Peroxide 30 %w/w of Hydrogen peroxide and thermal (105°C / 24 hours 7 minutes) ,photolytic degradation ( 321929 Lux hours & 97.02 Watt hours / sq. m at 25 °C). Keywords: Fusidic acid, Betamethasone -17 valerate, Chlorocresol, Forced degradation, HPLC, Method validation.

ABSTRACT A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Domperidone and Dexrabeprazole, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5μm) column using a mixture of Methanol: Phosphate Buffer pH 3.5 (65:35) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 270 nm. The retention time of the Domperidone and Dexrabeprazole was 2.456, 4.312 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25mg/ml of Domperidone and 2.5-12.5mg/ml of Dexrabeprazole. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. Keywords: Domperidone, Dexrabeprazole, RP-HPLC, validation.