ABSTRACT

Tamaka Shwasa is a Swatantra Vyadhi& having its own etiology, pathology & management. Its clinical feature resembles with the Bronchial asthma, a very distressing disease of respiratory system producing dyspnoea & discomfort, making life miserable. The GINA report 2012 says, Asthma is a serious global health problem. Vasadi Vati is mentioned in classical text of Yogratnakar in Shwasa chikitsa adhyay, which contains Vasa, Haridra, Dhanyaka, Guduchi, Bharangi, Pippali, Sunthi, Kantakari and Marich. Till date there is no reference regarding scientific analysis of Vasadi vati, so the present work was carried out to standardize the finished product Vasadi vati to conform its identity, quality and purity. The presence of Trichome, Aloerone grains, Epicarp cells along with oil globule, Simple and compound starch grains, Border pitted vessels, Collenchyma cells were the characteristic features observed in the microscopy of drug. Physico-chemical analysis shows water soluble extract is 31.1 % w/wand PH is 6.0. High Performance Thin Layer Chromatography (HPTLC) at 254nm and 366nm resulted into 10 & 9 spots respectively.

Keywords: Vasadi vati, Tamaka Shwasa, Pharmacognosy, HPTLC.

ABSTRACT A sensitive, precise and simple LC method for the simultaneous quantification of epigallocatechin-3-gallate and atorvastatin in rat plasma has been developed and validated. The chromatographic separation was achieved on a C18 column (250mm×4.6 mm, 5 μm) maintained at room temperature, using gradient elution with 0.1% formic acid (Solvent A) and acetonitrile (Solvent B), and detected using UV-visible detector. Protein precipitation followed by liquid-liquid extraction of epigallocatechin-3-gallate and atorvastatin from rat plasma resulted in their high recoveries. LC calibration curves based on the extracts from rat plasma were linear in the range of 30–1000 ng/ml for both the analytes. The limits of quantification were 30ng/ml for both epigallocatechin-3-gallate as well as atorvastatin. The precision and accuracy of the method were well within the generally accepted criteria for biomedical analysis. The described method was successfully applied to study the effect of epigallocatechin-3-gallate, which is reported as a P-glycoprotein inhibitor on the pharmacokinetics of atorvastatin (P-glycoprotein substrate) in Wistar rats. The results of the study inferred that epigallocatechin-3-gallate significantly improved the oral bioavailability of atorvastatin.

Keywords: Column liquid chromatography,P-glycoprotein, Epigallocatechin-3-gallate, Atorvastatin, Pharmacokinetics.

ABSTRACT The objective of the present study to develop and validation of Amlodipine Besylate, Olmesartan Medoxomil, Hydrochlorothiazide in bulk and in combined tablet formulation by UV Spectrophotometric method. Blood pressure lowering drugs such as Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorothiazide dissolved in methanol (1000g/ml). The stock solutions were further diluted with distilled water to get 10 g/ml scanned in the Double beam UV Spectrophotometer. Amlodipine Besylate estimated at 255 nm, 315 nm and 365 nm, Hydrochlorothiazide at 255 nm and 315 nm and Olmesartan Medoxomil at 255 nm were used and the absorbance corrected for interference method was applied. Hence, Hydrochlorothiazide was found at 315 nm and Olmesartan Medoxomil found at 255 nm. Calibration curve was plotted by using concentration versus absorbance. Range of Amlodipine Besylate was 1 – 5 g/ml. The absorbance of these solutions were measured at 365 nm, 315 nm and 255 nm respectively. Hydrochlorothiazide obeys Beer’s law in the range of 2.5 – 12.5 g/ml and measured at 255 nm and 315 nm. Whereas range of Olmesartan Medoxomil was 4 – 20 g/ml and measured at 255 nm. Correlation coefficient value of these drugs found to be 0.99992.The amount of Olmat AMH (Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil) tablets was found to be 99.78 ± 1.1367, 100.90 ± 0.7190 and 99.88 ± 0.5308 for Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil respectively. The % RSD (Relative Standard Deviation) values were found to be 1.1392, 0.7126 and 0.5315 for Amlodipine Besylate, Hydrochlorothiazide and Olmesartan Medoxomil respectively. The % RSD value of intraday and inter day analysis were found to be 1.7430 and 0.8715 for Amlodipine Besylate, 0.8361 and 0.6869 for Hydrochlorothiazide and 0.7288 and 0.6542 for Olmesartan Medoxomil. The accuracy of the method was performed by recovery studies. The percentage recovery was found to be in the range of 100.23– 101.27% for Amlodipine Besylate, 99.99 – 100.10% for Hydrochlorothiazide and 99.94 – 100.05% for Olmesartan Medoxomil. Keywords: UV Spectrophotometric Method, Amlodipine Besylate, Olmesartan Medoxomil, Hydrochlorothiazide.

ABSTRACT A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Isosorbide Dinitrite and Hydralazine HCl in pharmaceutical dosage form. Chromatographic separation was performed on Agilant Zorbax (C18) (4.6mm x 250mm, 5m) column, with mobile phase comprising of mixture of buffer (pH6.5, adjusted with potassium dihydrogen phosphate), acetonitrile in the ratio of 70:30 v/v, at the flow rate 0.8 ml/min. The detection was carried out at 274 nm. The retention times of Isosorbide Dinitrite and Hydralazine HCl were found to be 3.6 and 2.7 mins respectively with a run time of 6 mins, theoretical levels for Isosorbide Dinitrite and Hydralazine HCl were 8055 and 7525 respectively, with a resolution of 6.57. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation, robustness and ruggedness. Linearity of Isosorbide Dinitrite was found in the range of 50-150 μg/mL and that for Hydralazine HCl was found to be 50-150 μg/mL. The correlation coefficient for Isosorbide Dinitrite and Hydralazine HCl were 1 and 0.999 respectively. The LOD values for Isosorbide Dinitrite and Hydralazine HCl were 2.95 and 2.88 μg/mL respectively. The LOQ values for Isosorbide Dinitrite and Hydralazine HCl were and 9.8 and 9.6 μg/mL respectively. This demonstrates that the developed method is simple, precise, rapid, selective, accurate and reproducible for simultaneous estimation of Isosorbide Dinitrite and Hydralazine HCl tablet dosage form. Keywords: Isosorbide Dinitrate, Hydralazine HCl, RP-HPLC, Validation.

ABSTRACT This article provides an introduction to Fourier transform-based mass spectrometry. The key performance characteristics of Fourier transform-based mass spectrometry, mass accuracy and resolution, are presented in the view of how they impact the interpretation of measurements in proteomic applications. The theory and principles of operation of two types of mass analyzer, Fourier transform ion cyclotron resonance and Orbitrap, are described. Major benefits as well as limitations of Fourier transform-based mass spectrometry technology are discussed in the context of practical sample analysis, and illustrated with examples included as figures in this text and in the accompanying slide set. Comparisons highlighting the performance differences between the two mass analyzers are made where deemed useful in assisting the user with choosing the most appropriate technology for an application. Recent developments of these high-performing mass spectrometers are mentioned to provide a future outlook. Keywords: Mass spectrometry, Orbitrap, Laser desorption, Analyzers.

ABSTRACT Ultra high performance liquid chromatography\\ms is an extremely versatile instrumental technique. As the name suggested the instrumentation comprises a high performance liquid chromatography attached, via suitable interface, to a mass spectrometer. The primary advantage UHPLC/MS has over GC/MS is that it is capable of analyzing a much wider range of components. Compounds that are thermally liable, exhibit high polarity or have a high molecular mass may all be analyzed using UHPLC/MS even proteins may be routinely analyzed. Ultra high performance liquid chromatography (UHPLC) performs separations 5 to 10 times faster than conventional HPLC by employing sub-2 μm diameter particles. The 1-2 second peak widths and relatively high separation efficiency of UHPLC are more competitive with capillary GC, making UHPLC-MS an attractive alternative method for illicit drug analysis. The columns packed with porous sub-2μm particles and the extension of the upper pressure limit of HPLC instrumentation to 1300bar (ultra-high pressure liquid chromatography, UHPLC) has opened new frontiers in resolution and speed of analysis. However, certain constraints appear when coupling UHPLC technology with mass spectrometry (MS). Firs Chromatography (HPLC) directly coupled to mass spectrometry (MS) was in routine use in drug metabolism laboratories. It can give Enhanced selectivity and sensitivity, and rapid, generic analysis. Keywords: Ultra performance, Spectroscopy, Sensitivity, Ionization, Analiser.

ABSTRACT Flash chromatography is widely used for purification of low molecular weight natural compounds and products of organic synthetic reactions. Modern flash techniques include the use of convenient disposable flash cartridges instead of glass columns. Flash purification systems allow users to speed up the purification process for quicker results and higher throughput. In this review, we present the introduction of FC, the principle involved, instrumentation, general procedure and advancement in Flash chromatography along with its applications. Keywords: Flash cartridges, Molecular weight and Modern flash.

ABSTRACT Laser-induced breakdown spectroscopy (LIBS) is a type of atomic emission spectroscopy which uses a highly energeticlaser pulse as the excitation source. The laser is focused to form a plasma, which atomizes and excites samples. In principle, LIBS can analyse any matter regardless of its physical state, be it solid, liquid or gas. Because all elements emit light of characteristic frequencies when excited to sufficiently high temperatures, LIBS can (in principle) detect all elements, limited only by the power of the laser as well as the sensitivity and wavelength range of the spectrograph & detector. If the constituents of a material to be analyzed are known, LIBS may be used to evaluate the relative abundance of each constituent element, or to monitor the presence of impurities. In practice, detection limits are a function of a) the plasma excitation temperature, b) the light collection window, and c) the line strength of the viewed transition. LIBS makes use of optical emission spectrometry and is to this extent very similar to arc/spark emission spectroscopy. Keywords: Laser-induced, plasma excitation temperature, optical emission spectrometry.

ABSTRACT The present study is concerned with the determination of HDL & LDL (Lipoproteins) in the serum of patients with chronic renal disease. Fifty (50) patients with chronic renal disease and fifty (50) healthy controls were included in this study. The results obtained show a significant increased level of serum LDL levels whereas decreased HDL levels in patients with chronic renal disease patients as compared with control group.

Keywords: Lipoproteins, HDL, LDL, Chronic Renal Disease.

ABSTRACT A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for estimation of eletriptan in tablet dosage form. Chromatographic separation was carried out by using mobile phase 0.02M potassium dehydrogenate Phosphate buffer (ph 3.0) : Acetonitrile (50:50 v/v, PH-3.0 adjusted with Orthophosphoric acid ) on Hypersil, Symmetry C8 150×4.6mm, 5m at flow rate 0.8ml/min in isocratic mode and effluents are monitored at 221 nm. The retention time for eletriptan was found to be 2.45 min respectively and calibration curves were linear over concentration from 10-50μg/ml. The method was validated for accuracy, precision, specificity, linearity, system suitability. LOD and LOQ were 0.0258μg/ml and 0.0897μg/ml for eletriptan. The percentage recovery of eletriptan was found to be 98-102%.The developed method was fast, accurate, precise, and successfully applied to estimate the amount of eletriptan in bulk sample and tablet dosage form so it can be used for quality control department.

Keywords: Eletriptan, RP-HPLC, Hypersil column.