Mebeverine is an antispasmodic drug that is claimed to act directly on the colonic muscle and is virtually free of systemic adverse reactions. It has been used to treat irritable bowel syndrome, but its therapeutic effect is poor. Mebeverine exhibits the classical property of pH- dependent hydrolysis which is invariably associated with the ester functional group. Thus, neutral solutions of mebeverine are quite stable, but at ambient temperature the drug degrades in either 0.5 M HCL and 0.1 M NaOH. Half-lives of 90 and 0.2 days respectively were measured in these systems. This review highlights different analytical methods such as chromatography, spectroscopy, and hyphenated techniques of Mebeverine hydrochloride. These techniques are either explored for the quantification, detection of metabolite and also for stability-studies of the Mebeverine hydrochloride. The present studies revealed that HPLC techniques along with spectroscopic have been most widely explored for the analysis. The brief review may provide information to the researchers who are working in the area of analytical research of Mebeverine hydrochloride

The present study was aimed at developing ?Extraction, method development and Analytical validation of crude and marketed drugs by using UV Spectroscopy method. In order to effect analysis of the component peaks, different suitable compound is tested by using different polar and non-polar solvents like Dimethyl Sulfoxide, Benzene, Acetone, Ethyl acetate, Ethanol, Methanol, Diethyl ether, distilled water etc. Extractions of different compounds are carried out using different solvents like Ethanol for Sage, Ethyl acetate for basil, Distilled water for Garlic. Extraction is done by using Soxhelet apparatus. For this process different quantities components of are taken like Sage Powder (96,8g), Basil powder (15.7g), Garlic (90g). In this process the powders are placed in a Soxhelet apparatus. 200ml of each solvent is taken for the extraction of each plant component. Before the extraction process, the plant parts are taken in required quantities like Sage leaves (200g), Basil leaves (37.2g), Garlic (250g) are dried under sunlight in shade. Along with these plant products, marketed drugs like Lopinavir and Efavirenz are taken in the quantity of 20 tablets each. After that extracted plant products aremade into dilutions and analyzed by using UV-spectrophotometer. The Sage is analyzed between 370nm-590nm; the ? max of the Sage is 580nm. The Basil is analyzed between 385nm-715nm; there are 2 ? max for Basil—Blue color obtained at 435nm and Red light obtained at 665nm. The Garlic is analyzed between 200nm-400nm; the ? max is 237nm. The Lopinavir is analyzed between 200nm-350nm; the ? max is obtained at 284nm. The Efavirenz is analyzed between 200nm-300nm; the ? max is obtained at 248nm. All these values are noted and graph is draw

The main purpose of the study was to develop accurate, precise and economic methods for the determination of Dutasteride HCL and Alfuzosin. An HPLC method is developed and validated for various parameters as per ICH guidelines. The system suitability parameters prove that the proposed method is equally suitable for estimation of Dutasteride HCL and Alfuzosin, the chromatogram for Dutasteride and Alfuzosin were found to be satisfactory. The retention time for Dutasteride HCL about 5min, and Alfuzosin was about 2min. The sensitivity of the method is good and also linearity which is observed is good. The accuracy of the method is determined by recovery of drug is well within the acceptance limits of 97-103%. The method is rugged and robust as observed from insignificant variation in the results of analysis on changes in mobile phase composition ratio, pH, flow rate, temperature and analysis being performed by different analysts and on different days respectively. In the all above cases the recovery is found to be within the limit