Ultra violet absorption spectra arise from transition of electrons within a molecule from a lower level to a higher level. A molecule absorb ultraviolet radiation of frequency (?), the electron in that molecule undergo transition from lower to higher energy level. The energy can be calculates by the equation.E1- E0 = h?. Method validation is the process of demonstrating that analytical procedures are suitable for their intended use and that they support the identity, quality, purity and potency of the drug substances and drug products. Simply, method validation is the process of proving that an analytical method is acceptable for its intended purpose

Hydrazone and acylhydrazone derivatives have been considerable interest in the development of novel compounds with anticonvulsant, antiinflammatory, antidepressant, analgesic, antiplatelet, antimalarial, antimicrobial, antimycobacterial, anticancer activities. Hydrazones containing an azometine –NHN=CH- proton are synthesized by heating the appropriate substituted hydrazines/hydrazides with aldehydes and ketones in solvents like ethanol, methanol, tetrahydrofuran, butanol, glacial acetic acid, ethanol-glacial acetic acid. Hydrazide-hydrazones compounds are not only intermediates but also very effective organic compounds in their own right. When they are used as intermediates, coupling products can be synthesized by using the active hydrogen component of –CONHN=CH- azometine group