The present study was aimed to investigate the antimicrobial, antioxidant activity and HPTLCfinger print profile of methanolic extract of Hibiscus radiatus (MEHR) and Terminalia arjuna (META) .HPTLC finger print profile was showed the active compounds present in crude extracts, which may responsible for the antioxidant and antimicrobial prospective. Antioxidant bioautography shows the strong antioxidant activity of one flavonoid band, whereas no activity detected of bitter principles. Bioautography showed that the antimicrobial and antioxidant activity was probably due to flavonoids. These results showed that, the significant antimicrobial properties against pathogen; this work will be helpful to explore the active compound identification in the field of pharmaceutical research and able to produce new drug molecules against pathogens.