The assay of an absorbing substance may be quickly carried out by preparing a solution in a transparent solvent and measuring its absorbance at a suitable wavelength. The wavelength normally selected is a wavelength of maximum absorption (? max), where small errors in setting the wavelength scale have little effects on the measured absorbance. Aim of the present study is to develop an accurate, precise, sensitive, reproducible, analytical technique and forced degradation study to determine the Voclosporin in pharmaceutical dosage forms. Voclosporin is a calcineurin inhibitor for the treatment of lupus nephritis (LN). Lupus nephritis (LN) is a type ofglomerulonephritis occurring in patients with systemic lupus erythematosus (SLE). The linearity data for Voclosporin is given in capsule; the linearity plots of Voclosporin at 222 nm. The regression equation and correlation coefficient forthe calibration cure of Voclosporin at 222 nm were found linear in the concentration range of 1.9 - 11.8 µg/mL at wavelength. Simple, precise and accurate UV spectrophotometric method were developed and validated as per ICH guidelines for the estimation of Voclosporin in capsule dosage form.

Nystatin is an antifungal medication. It is used to treat Candida infections of the skin including diaper rash, thrush, esophageal candidiasis, and vaginal yeast infections. It may also be used to prevent candidiasis in those who are at high risk. Nystatin may be used by mouth, in the vagina, or applied to the skin. The aim of the present study is to formulate and evaluate microspheres loaded transdermal gel containing Nystatin as a model drug microsphere were prepared by using aqueous ionotropic gelation method. Different polymers, different drug to polymer(s) ratio(s) and other parameters were screened to study their effects on properties of microspheres and to optimize each parameter. The microspheres obtained were subjected to preformulation studies like bulk density, tapped density, angle of repose, carr’s index, hausner’s ratio the results obtained were within the limit. The microspheres were characterized by Percentage yield, Drug entrapment efficiency, Particle size analysis, then the optimized microspheres formulation F3 were incorporated into the gel prepared with various grades of carbopol polymer(s) ratio(s) and was evaluated by parameters like Visual inspection , pH measurement , Spreadability studies , Viscosity and invitro drug release by using franz diffusion cell for results from the diffusion results F5 showed maximum percentage drug release of 96.72 hence it was considered as the optimized formulation

New method was established for simultaneous estimation of Bempedoic acid and Ezetimibe by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Bempedoic acid and Ezetimibe by using ACE C18 column (4.6×150mm) 5µ, flow rate was 1.2 ml/min, mobile phase ratio was (70:30 v/v) methanol:Phosphate buffer pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength was 240nm. The instrument used was Shimadzu, model No. SPD-20MA LC+20AD, Software- LC-20 Solution. The retention times were found to be 2.733 mins and 3.415 mins. The % purity of Bempedoic acid and Ezetimibe was found to be 101.27% and 99.97% respectively. The precision study was precise, robust, and repeatable. LOD value was 3.17 and 5.68, and LOQ value was 0.0172 and 0.2125 respectively. Hence the suggested RP-HPLC method can be used for routine analysis of Bempedoic acid and Ezetimibe in API and Pharmaceutical dosage form