ABSTRACT

Conventional oral formulations like solution, suspension, and ointments have many disadvantages which result into poor bioavailability of drug in the buccal cavity. The poor bioavailability and therapeutic response may be overcome by the use of mucoadhesive in situ gel forming systems that are applied as liquid into the buccal cavity and undergo a sol-gel transition which have good mucoadhesion with buccal mucus layers. The objective of this study was to formulate and evaluate in situ oral topical gels of Miconazole based temperature induced systems for the treatment of oral condidiasis.Mucoadhesive systems were prepared by using polaxamer 188 combined with carbapol 934to enhance the gel bio adhesion properties. Increase in the concentration of mucoadhesive agent enhanced the mucoadhesive force significantly. In vitro release of fluconazole from the mucoadhesive system in simulated salivary fluid was influenced significantly by the properties and concentration of carbapol 934 showed to enhance bioavailability through its longer oral residence time and ability to sustain the release of the drug. Significant reduction in the total bacterial count was observed between drug solution (control) and mucoadhesive batches against both tested organisms. 

Keywords: Mucoadhesive, Miconazole, Polaxamer, Carbapol, Thermoreversible insitu gel, Oral drug delivery.

ABSTRACT

A simple, accurate, precise, economical and reproducible method was developed for simultaneous estimation of olmesartan, amlodipine and hydrochlorothiazide in bulk and its dosage form. The excipients in the commercial tablet preparation did not interfere with the assay. Here we have developed a validated simultaneous estimation of olmesartan, amlodipine and hydrochlorothiazide in bulk and its tablet formulation. The stock solutions were prepared in methanol followed by the further required dilutions with Double distilled water. The λmax for olmesartan, amlodipine and hydrochlorothiazide were 256.5 nm, 239 nm and 271.5nm respectively. Linearity in concentration range of 4-24 µg/ mL, 1-10 µg/ mL and 2-20 µg/mL was shown respectively by the three drugs. The proposed method has estimated olmesartan 99.45±0.94%, amlodipine 98.95±0.32% and hydrochlorothiazide 100.46±0.68% in marketed tablets. The results of analysis have been validated statistically and also by recovery studies. Validation of the proposed methods was carried out for its accuracy, precision, and ruggedness according to ICH guidelines. Thus the present study gives an excellent method for the determination of all the three drugs in combined dosage formulation without their prior separation. 

Keywords:  Olmesartan medoxomil, Amlodipine besylate, Hydrochlorothiazide, Method validation, Simultaneous estimation, validation.

ABSTRACT

The objective of this study was to formulate and evaluate in situ oral topical gels of Fluconazole based temperature induced systems for the treatment of oral condidiasis.Conventional oral formulations like solution, suspension, and ointments have many disadvantages which result into poor bioavailability of drug in the buccal cavity. The poor bioavailability and therapeutic response may be overcome by the use of mucoadhesivein situ gel forming systems that are applied as liquid into the buccal cavity and undergo a sol-gel transition which have good mucoadhesion with buccal mucus layers. Mucoadhesive systems were prepared by using polaxamer 188 combined with carbapol 934 to enhance the gel bio adhesion properties. Increase in the concentration of mucoadhesive agent enhanced the mucoadhesive force significantly. In vitro release of Fluconazole from the mucoadhesive system in simulated salivary fluid was influenced significantly by the properties and concentration of carbapol 934 showed to enhance bioavailability through its longer oral residence time and ability to sustain the release of the drug.

Keywords: Mucoadhesive, Fluconazole, Polaxamer, Carbapol, Thermoreversibleinsitu gel, Oral drug delivery.

ABSTRACT

Aleurites moluccana L. is used in folklore medicine in fomenting, sealing of secondary infection, healing of wounds and skin eruptions. There was no scientific evidence justifying the use of bark of Aleurites moluccana, therefore the present study was aimed at evaluation of wound healing activity of the plant. In the present study the bark of Aleurites moluccana  were studied for wound healing activity by incorporating extract in simple ointment base B.P. in concentration of 2% (w/w) and 4% (w/w) .Wound healing activity was studied in three types of model in rats viz. excision, incision and burn wound model. The results were also comparable to those of a standard drug, nitrofurazone in terms of wound contracting ability, wound closure time, tensile strength. The statistical data indicated that the wound with ointment containing 4% w/w alcoholic extract exhibited significant (P < 0.001) wound contracting ability and period of epithelization. Significant tensile strength was observed with both the ointment formulations 2% w/w and 4% w/w. The results of histopathological examination supported the outcome of both excision and burn wound models. The experimental data demonstrated that Aleurites moluccana displayed remarkable wound healing activity.

Keywords: Aleurites moluccana; Alcoholic extract; Excision wound model; Incision wound model; Burn wound model; Nitrofurazone.

ABSTRACT

A simple, precise and accurate stability indicating RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Valsartan and Atorvastatin from their combination dosage form. Water’s HPLC equipped with UV-Visible and Diode Array detectors, with Empower software was used. Column used was Hypersil BDS C18, 5 μm, 250 mm × 4.6 mm i.d., at 40°C. Mobile phase consisting of a mixture of 0.1% acetic acid and acetonitrile in the ratio 50:50 v/v respectively at a flow rate of 2.0 mL/ min and UV detection was carried out at 225 nm for Valsartan and 246 nm for Atorvastatin. Valsartan and Atorvastatin in their combined dosage form were exposed to thermal, photolytic, oxidative, acid-base hydrolytic stress conditions, the stressed samples were analyzed by proposed method. Peak purity results suggested no other co-eluting, interfering peaks from excipients, impurities, or degradation products due to variable stress condition, and the method is specific for the estimation of Valsartan and Atorvastatin in presence of their degradation products and impurities within 7 minutes. The retention time of Valsartan and Atorvastatin were 3.33 and 5.44 minutes respectively. The method was found linear over the range of 5-15 μg per mL for Atorvastatin and 40-120 μg per mL for Valsartan. The proposed method was validated as per the ICH and USP guidelines. 

Key words: Valsartan, Atorvastatin, RP-HPLC method.